What is colometric assay?

Colorimetric assay is a type of biochemical test used to quantify the concentration of a specific substance in a solution based on its color change. This assay relies on a chemical reaction that produces a colored compound when the target molecule is present in the solution. The intensity of the color is proportional to the concentration of the analyzed substance in the sample.

Colorimetric assays are used in various scientific fields, including medicine, food science, environmental monitoring, and biotechnology. They are easy to perform, rapid, and cost-effective, making them ideal for high-throughput screening of large sample quantities. Colorimetric assays are commonly used to measure enzymatic activity, metal ion concentration, protein quantification, and cell viability. Examples of commonly used colorimetric assays include the Bradford assay, the Lowry protein assay, and the MTT assay.